Kenneth Matreyek, Ph.D.
kmatreyek[at]gmail.com / matreyek[at]uw.edu
University of Washington, Department of Genome Sciences
Foege Building Room S-041, Box 355065
3720 15th Ave NE, Seattle, WA, 98195
Harvard University, Cambridge, MA
Ph.D. in Virology
9/2006 – 11/2013
Dissertation: HIV-1 capsid engages nucleoporin NUP153 to promote viral nuclear entry
Date of dissertation defense: 10/4/2013, Date of degree: 3/7/2014
Major fields: Virology, Molecular Genetics, Biochemistry, Cell Biology
University of California Los Angeles, Los Angeles, CA
B.S. with Honors in Microbiology, Immunology & Molecular Genetics
9/2002 – 6/2006
Magna Cum Laude.
Case Western University School of Medicine, Cleveland, OH
Department of Pathology
9/2019 – present
University of Washington, Seattle, WA
Department of Genome Sciences
5/2014 – 8/2019
American Cancer Society Postdoctoral Fellow in the laboratory of Douglas Fowler, Ph.D.
Dana-Farber Cancer Institute, Boston, MA
Department of Cancer, Immunology and AIDS
12/2013 – 4/2014
Research Fellow in the laboratory of Alan Engelman, Ph.D.
Harvard Medical School at the Dana-Farber Cancer Institute, Boston, MA
Program in Virology & Department of Cancer, Immunology and AIDS
11/2007 – 11/2013
Graduate student in the laboratory of Alan Engelman, Ph.D.
PDL BioPharma, Fremont, CA
Intern under the direction of Dr. Audie Rice, Ph.D.
University of California Los Angeles, Los Angeles, CA
Department of Microbiology, Immunology and Molecular Genetics
5/2004 – 6/2006
Research honors thesis in the laboratory of Benhur Lee, Ph.D.
Matreyek KA°, Stephany JJ, Chiasson MA, Hasle N, Fowler DM. An improved platform for functional assessment of large protein libraries in mammalian cells. Nucleic Acids Res. 2019 Oct; http://doi.org/10.1093/nar/gkz910 ° co-corresponding
Matreyek KA*, Starita LM*, Stephany JJ, Martin B, Chiasson MA, Gray VE, Kircher M, Khechaduri A, Dines JN, Hause RJ, Bhatia S, Evans WE, Relling MV, Yang W, Shendure J, Fowler DM. Multiplex assessment of protein variant abundance by massively parallel sequencing. Nat Genet. 2018 Jun; 50(6):874-882 *contributed equally
Hill AJ, McFaline-Figueroa JL, Starita LM, Gasperini MJ, Matreyek KA, Packer J, Jackson D, Shendure J, Trapnell C. On the design of CRISPR-based single cell molecular screens. Nat Methods. 2018 Apr; 15(4):271-274.
Matreyek KA, Stephany JJ, Fowler DM. A platform for functional assessment of large variant libraries in mammalian cells. Nucleic Acids Res. 2017 Jun;15(4):e102.
Matreyek KA, Wang W, Serrao E, Singh P, Levin HL, Engelman A. Host and viral determinants for MxB restriction of HIV-1 infection. Retrovirology. 2014 Oct; 11:90.
Fribourgh JL*, Nguyen HC*, Matreyek KA*, Alvarez FJD, Summers BJ, Dewdney TG, Shi J, Aiken C, Zhang P, Engelman A, Xiong Y. Structural insight into HIV-1 restriction by MxB. Cell Host Microbe. 2014 Nov; 16:1-12. * contributed equally
Matreyek KA, Yücel SS, Li X, Engelman A. Nucleoporin NUP153 phenylalanine-glycine motifs engage a common binding pocket within the HIV-1 capsid protein to mediate lentiviral infectivity. PLoS Pathog. 2013 Oct;9(10):e1003693.
Koh Y, Wu X, Ferris AL, Matreyek KA, Smith SJ, Lee K, KewalRamani VN, Hughes SH, Engelman A. Differential effects of human immunodeficiency virus type 1 capsid and cellular factors nucleoporin 153 and LEDGF/p75 on the efficiency and specificity of viral DNA integration. J Virol. 2013 Jan;87(1):648-58.
Matreyek KA, Engelman A. The requirement for nucleoporin NUP153 during human immunodeficiency virus type 1 infection is determined by the viral capsid. J Virol. 2011 Aug;85(15):7818-27.
Koh Y, Matreyek KA, Engelman A. Differential sensitivities of retroviruses to integrase strand transfer inhibitors. J Virol. 2011 Apr;85(7):3677-82.
Krishnan L*, Matreyek KA*, Oztop I*, Lee K, Tipper CH, Li X, Dar MJ, Kewalramani VN, Engelman A. The requirement for cellular transportin 3 (TNPO3 or TRN-SR2) during infection maps to human immunodeficiency virus type 1 capsid and not integrase. J Virol. 2010 Jan;84(1):397-406. * contributed equally
Aguilar HC, Matreyek KA, Choi DY, Filone CM, Young S, Lee B. Polybasic KKR motif in the cytoplasmic tail of Nipah virus fusion protein modulates membrane fusion by inside-out signaling. J Virol. 2007 May;81(9):4520-32.
Aguilar HC, Matreyek KA, Filone CM, Hashimi ST, Levroney EL, Negrete OA, Bertolotti-Ciarlet A, Choi DY, McHardy I, Fulcher JA, Su SV, Wolf MC, Kohatsu L, Baum LG, Lee B. N-glycans on Nipah virus fusion protein protect against neutralization but reduce membrane fusion and viral entry. J Virol. 2006 May;80(10):4878-89.
Matreyek KA, Engelman A. Viral and cellular requirements for the nuclear entry of retroviral preintegration nucleoprotein complexes. Viruses. 2013 Oct 7;5(10):2483-511. doi: 10.3390/v5102483.
Matreyek KA*, Oztop I*, Freed EO, Engelman A. Viral latency and potential eradication of HIV-1. Expert Rev Anti Infect Ther. 2012 Aug;10(8):855-7. * contributed equally
GRANTS AND AWARDS
Finalist, UW Postdoc Mentoring Award (2019), 5/2019
National Institutes of Health (NIH) National Institute of Allergy and Infectious Diseases (NIAID) Career Transition (K22) Phase I Award Notification, 6/2018
Finalist, UW Postdoc Mentoring Award (2018), 4/2018
American Cancer Society Postdoctoral Fellowship (PF-15-221-01), 1/2016
University of Washington / Fred Hutchinson Cancer Research Center (FHCRC) Interdisciplinary Training Grant in Cancer (2T32CA080416),12/2014
Wasserman Scholarship – Undergraduate Research Scholarship Program, 9/2005